E X T O X N E T
Extension Toxicology Network
A Pesticide Information Project of Cooperative Extension Offices of
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University of California at Davis. Major support and funding was provided
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Pesticide
Information
Profile
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Methoprene
Publication Date: 9/95
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TRADE OR OTHER NAMES
Some trade names include Altosid, Apex, Diacon, Dianex, Kabat, Minex,
Pharorid, Precor, ZR-515.
REGULATORY STATUS
Methoprene is classified by the U.S. Environmental Protection Agency
(EPA) for general use as both an insecticide and a growth regulator. Products
containing methoprene must bear the signal word "Caution" (14). Check with
specific state regulations for local restrictions which may apply.
INTRODUCTION
Methoprene is referred to as an insect growth regulator because it
interferes with the maturation stages through which an insect goes: from egg,
larvae, and pupa, to adult. Growth regulators make it impossible for insects
to mature to the adult stage of development. Methoprene is considered a
biochemical pesticide because rather than controlling target pests through
direct toxicity, it interferes with the insects' life cycle and prevents them
from reaching maturity or reproducing (2, 16). In order to be effective, it
is essential that this growth inhibitor be administered at the proper stage of
the target pest's life cycle (4). Methoprene is not toxic to the pupal or
adult stages. Treated larvae will pupate but adults do not emerge from the
pupal stage. Methoprene is also considered a larvicide since it is effective
in controlling the larval stage of insects. Methoprene is used in the
production of a number of foods including meat, milk, eggs, mushrooms,
peanuts, rice and cereals. It is also used in aquatic areas to control
mosquitoes and several types of ants, flies, lice, moths, beetles and fleas
(7, 8, 16). It is available in liquid, solid and aerosol formulations (14).
TOXICOLOGICAL EFFECTS
ACUTE TOXICITY
Methoprene is relatively non-toxic when ingested or inhaled and slightly
toxic by dermal absorption. No overt signs of poisoning have been reported in
incidents involving accidental human exposure to methoprene (9). Methoprene
is not an eye or skin irritant and it is not a skin sensitizer. It poses very
little hazard to humans or other nontarget species with the exception of
estuarine invertebrates (16).
The amount of methoprene that is lethal to one-half (50%) of exposed test
animals is called its lethal dose fifty, or LD50. The oral LD50 for
methoprene in rats is greater than 34,600 milligrams per kilogram (mg/kg).
The oral LD50 for methoprene in dogs is between 5,000 and 10,000 mg/kg. The
dermal LD50 for methoprene in rabbits is between 3,038 - 10,250 mg/kg (18).
The lethal concentration fifty (LC50) is that concentration of a material
in air or water that kills half of a population that is experimentally exposed
to the chemical for a given time period. The inhalation LC50 for methoprene
in rats is greater than 210 mg/liter (1, 9). Labels on methoprene containers
bear the signal word "CAUTION" (2).
CHRONIC TOXICITY
No methoprene-related effects were observed in two-year feeding trials
with rats given up to and including a 5,000 ppm diet, nor in mice on a 250 ppm
diet, daily for 18 months (18).
Reproductive Effects
Experimental data indicate that there are no reproductive hazards
associated with methoprene (9, 16). No methoprene-related effects were
observed in three-generation reproduction studies in rats on 2,500 mg/kg diets
(10). Similarly, no effects were observed in the reproduction of bobwhite
quail and mallard ducks at 30 parts per million (ppm) constant feeding of
Altosid (11).
Teratogenic Effects
There are no teratogenic hazards associated with methoprene (9, 16). No
teratogenic effects were seen in rats or rabbits at 500 mg/kg (10, 17).
Mutagenic Effects
Methoprene is not mutagenic (16). No methoprene-related mutagenic
effects were observed in rats at 2,000 mg/kg (10, 17). This dose was given in
a single acute experiment and in five-day repeated dosing experiments (11).
Carcinogenic Effects
Methoprene is not a carcinogenic compound (16). No tumors were formed in
mice being fed daily diets containing methoprene up to and including 2,500
ppm, the highest dose tested, giving an approximate methoprene intake of 357
mg/kg/day in mice. In rats, the highest dose tested with no evidence of a
carcinogenic effect was 5,000 ppm in diet giving a daily methoprene intake of
approximately 250 mg/kg. NOELs (No-Observable-Effect-Level) for
carcinogenicity in rats or mice, if any, are higher than the highest dose
levels tested in these species. (18, 22)
Organ Toxicity
Liver changes were observed in mice fed diets containing methoprene at
1,000 and 2,500 ppm levels for 18 months (approximately equal to daily intakes
of 143 and 357 mg/kg). Increased liver weights were observed in rats that
were fed diets containing methoprene at 5,000 ppm (approximately equal to
daily intake of 250 mg/kg) for 90 days but not when rats were fed the 5,000
ppm diet daily for 24 months, allowing approximately the same intake of
methoprene (250 mg/kg/day). (18, 22)
Fate in Humans and Animals
In mammals, methoprene is rapidly and completely broken down and
excreted, mostly in the urine and feces (1, 4, 16). Some evidence suggests
that methoprene breakdown products, or 'metabolites,' are incorporated into
natural body components (9). Methoprene is excreted unchanged in cattle feces
in amounts that are sufficient to kill some fly larvae that breed in dung (4).
ECOLOGICAL EFFECTS
The use of methoprene as a mosquito larvicide should have no adverse
effects on populations of endangered birds, mammals, or fish (9). However, it
may kill shrimp and crabs (8), but the dose levels of Altosid Liquid Larvicide
(A.L.L.), an (S)-methoprene based mosquito control product, currently on the
market, required to produce such an effect is 105 times higher than the usual
level of 10 ppb, generated by recommended rate for A.L.L. application (18).
Effects on Birds
According to a recent EPA evaluation of ecological effects, methoprene is
considered practically non-toxic to mallard duck (16). This is because oral
LD50 of methoprene for mallard is greater than 2,250 mg/kg with no mortality
or any overt signs of toxicity at any dose level. Further, LC50 in 8-day
feeding studies in both, mallard duck and bobwhite quail was more than 10,000
ppm, indicating again that methoprene is practically non-toxic to birds (3, 18, 21).
Methoprene had no effect on quail reproduction (16).
Effects on Aquatic Organisms
Methoprene is moderately toxic to warm water, freshwater fish, and is
slightly toxic to cold water, freshwater fish (16). Exposure of aquatic
organisms will be limited by the low solubility (0.51 ppm) of methoprene in
water and by its rapid degradation in aquatic environments.
The level of Altosid in water that was lethal for 50% of the test fish
was 4.62 ppm in bluegill, 4.39 ppm in trout, and greater than 100 ppm for
channel catfish and largemouth bass (14). Methoprene residues build up in the
edible tissues of bluegill sunfish and crayfish (9).
Technical methoprene can be very highly acutely toxic to estuarine and
marine invertebrates. Marine organisms are not likely to be exposed as a
result of the use of methoprene as a mosquito larvicide (16). The LC50 of
methoprene for fresh water shrimp was greater than 100 ppm. The LC50 for
estuarine mud crabs was greater than 0.1 ppm (12). The effects of a
methoprene based mosquito larvicide product that is actually used in the field
(ALTOSID LIQUID LARVICIDE - A.L.L.), on the non-target aquatic crustaceans,
such as adults and larvae of shrimp, crabs and adult ghost shrimp have been
investigated under controlled conditions, only recently. These data indicate
that a 96-hour LC50 for adult shrimp or adult crab was 105 times higher than
the levels of 10 ppb, generally expected to be produced at recommended
application rate of A.L.L. For larvae, the 48-hour LC50 was 103 times higher
than the concentration of methoprene measured in water (2.5 ppb) at the
recommended rate of A.L.L. application (19). Except when applied in slow-
release briquettes, use of methoprene is not expected to result in exposure to
aquatic invertebrates because methoprene is short-lived in aquatic environment
and it does not have a high potential for bioaccumulation. Data regarding the
use of briquette formulation is currently under review by the EPA (15),
however, data provided by the manufacturer suggests that even briquettes do
not produce methoprene levels higher than 10 ppb at any time (18, 20).
Effects on Other Animals (Nontarget species)
Methoprene is toxic to amphibians, such as frogs, toads, and salamanders.
Reportedly, low application rates and rapid dissipation characteristics of
this material should prevent water concentrations from reaching levels that
are lethal to most amphibians. Methoprene use as mosquito larvicide poses
some hazards to freshwater invertebrates, but major effects are unlikely (9).
Methoprene poses little danger to nontarget insects. It is likely,
however, that Altosid could negatively affect some nontarget insects if it is
applied at rates that far exceed those intended for use (12). While it is
toxic to most insects, there is a distinct difference among the
susceptibilities of various species to the adverse effects of this material.
The critical factor is the timing of treatment in relation to the insect's
stage of development (4). It can be used around bees with minimum injury (6).
Altosid had very little effect, if any, on 35 species of exposed nontarget
organisms including earthworms, waterfleas, damselflies, snails, tadpoles, and
mosquito fish (12).
ENVIRONMENTAL FATE
Breakdown of Chemical in Soil and Groundwater
Methoprene is not persistent in soils and is unlikely to contaminate
groundwater (2, 16). The breakdown, or degradation, of methoprene was rapid
in experimental soil tests. In soil, microbial degradation is rapid and
appears to be the major route of its disappearance from soil (9, 16).
Methoprene also degrades rapidly in sunlight (16). In sandy loam, its half-
life was calculated to be about 10 days (5). When Altosid was applied at an
extremely high application rate of one pound per acre, its half-life was less
than ten days. Methoprene is not likely to leach, since it is rapidly bound,
or adsorbed, to soil. It was relatively immobile in four experimental soil
types (9). In leaching studies, Altosid has been observed only in the top few
inches of the soil, even after repeated washings with water (11).
Breakdown of Chemical in Water
Methoprene degrades rapidly in water (16). Studies have been conducted
in a pond on the degradation of methoprene by microorganisms. The
insecticide's half-life in pond water was about 30 hours at 0.001 ppm and 40
hours at 0.01 ppm (5). Under normal conditions of sunlight and temperature,
technical methoprene rapidly degrades when it is applied to water. The half-
life of this material is less than two days in the field. Aquatic
microorganisms and sunlight degrade Altosid (12). Methoprene degrades rapidly
in sunlight, both in water and on inert surfaces (16). Since methoprene is
toxic to aquatic invertebrates, it should be used carefully and in accordance
with label directions. Water can be contaminated and aquatic organisms harmed
by inappropriate cleaning of equipment, or disposal of wastes, associated with
methoprene (9).
Breakdown of Chemical in Vegetation
The half-life of methoprene on wheat was estimated to be three to seven
weeks, depending on the level of moisture in the plant (5). Studies have
shown that wheat grown in treated soil did not contain residues of methoprene.
Methoprene is biodegradable and nonpersistent even in plants treated with very
high rates. It has a half-life of less than two days in alfalfa when applied
at a rate of one pound per acre. In rice, the half-life is less than one day
(12).
PHYSICAL PROPERTIES AND GUIDELINES
Technical methoprene is an amber or pale yellow liquid with a faint
fruity odor (9, 14).
Exposure Guidelines:
| NOEL: | 250 ppm for systemic toxicity, based on an 18-month oncogenicity study (9). |
| PADI: | 0.0063 mg/kg/day based on a NOEL of 500 ppm in a 3-month dog feeding study using a 2,000-fold safety factor (17). |
| MPI: | 0.3750 mg/day for a 60 kg person (17). |
Physical Properties:
| CAS #: | 40596-69-8 |
| Specific gravity: | 0.9261 g/ml at 20 degrees C (14) |
| H20 solubility: | practically insoluble, 0.51 ppm |
| Solubility in other solvents: | soluble in organic solvents (2) |
| Boiling point: | 100 degrees C at 0.05 mm Hg (14) |
| Vapor pressure: | 2.37 x 10 to the minus 5 mm Hg at 25 degrees C (14); 1.60 x 10 to the minus 4 mm Hg at 40 degrees C (9, 14) |
| Chemical Class/Use: | insect growth regulator; synthetic juvenile hormone; selective mosquito larvicide |
BASIC MANUFACTURER
Sandoz Agro. Inc.
1300 E. Touhy Ave.
DesPlaines, IL 60018
Telephone: 708-390-3664
Review by Basic Manufacturer:
Comments solicited: October, 1992
Comments received: February, 1995
REFERENCES
Berg, G. L., ed. 1986. Farm chemicals handbook. Willoughby, OH:
Meister Publishing Co.
_____. 1984. Farm chemicals handbook. Willoughby, OH: Meister
Publishing Company.
Hudson, R. H., et al. 1984. Handbook of toxicity of pesticides to
wildlife. Second edition. United States Department of the Interior. Fish
and Wildlife Service. Resource Publication 153. Washington, DC.
McEwen, F. L. and G. R. Stephenson. 1979. The use and significance of
pesticides in the environment. NY: John Wiley and Sons, Inc.
Menzie, C. M. 1980. Metabolism of pesticides. Update III. U.S.
Department of the Interior. Fish and Wildlife Service. Special Scientific
Report. Wildlife No. 232. Washington, DC: U.S. Government Printing Office.
Morse, R. A. 1987. Bee poisoning. 1988 New York State pesticide
recommendations. Forty-ninth annual pest control conference. Nov. 9, 10, 11.
Cornell University. Ithaca, NY.
National Pest Control Association, Inc. 1980 (Nov.). Technical release
on new pesticide - Precor 5E. Vienna, VA.
Thomson, W. T. 1976. Insecticides, acaricides and ovicides.
Agricultural Chemicals, Book I. Indianapolis, IN: Thomson Publications.
U.S. Environmental Protection Agency. 1982 (Mar.). Guidance for the
reregistration of pesticide products containing methoprene as the active
ingredient. Office of Pesticide Programs, Registration Division. Washington,
DC.
Worthing, C. R., ed. 1983. The pesticide manual: A world compendium.
Croydon, England: The British Crop Protection Council.
Zoecon Corporation. 1974. Technical bulletin on Altosid.
Toxicological properties.
_____. 1973. Technical bulletin on Altosid. Environmental properties.
Hayes, W. J. and E. R. Laws (ed.). 1990. Handbook of Pesticide
Toxicology, Vol. 3, Classes of Pesticides. Academic Press, Inc., NY.
Meister, R. T. (ed.). 1992. Farm Chemicals Handbook '92. Meister
Publishing Company, Willoughby, OH.
US Environmental Protection Agency. 1991 (June 12). Pesticide
Reregistration Eligibility Document for methoprene; Availability for comment.
Federal Register 56 (113): 27017-8.
_____. 1991 (March). R.E.D. Facts: Methoprene. Pesticides and Toxic
Substances, US EPA, Washington, DC.
_____. 1981 (Dec. 4). Methoprene; Tolerances and exemptions from
tolerances for pesticide chemicals in or on raw agricultural commodities.
Federal Register 46 (233): 59248-9.
Review by Sandoz Agro. February, 1995.
Mortimer, M. R. and H. F. Chapman. 1994. A comparison of the toxic
effects of Altosid® Liquid Larvicide (S-methoprene) and Abate® (temephos) to
some non-target aquatic crustacean species common in eastern Australia.
Griffith University, Queensland, Australia.
Judy, D. and B. Howell. 1993. Concentrations of methoprene found in
freshwater microcosms treated with sustained release Altosid formulations.
Zoecon Study #1540. ABC Final Report #39541, MRID #42811202.
US Environmental Protection Agency. December, 1994. Pesticide
rejection rate analysis -- ecological effects. Washington, DC. EPA Doc. 738-
R-94-035.
Lehman, A. J. 1954. Approximate relation of parts per million in the
diet to mg/kg D.W./day. Association of Food and Drug Officials Quarterly
Bulletin 18:66.
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in nature and may no longer be applicable.
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